Microbiological testing

The safety of food is a priority not only to consumers and manufacturers, but also to legislative bodies. That is why laws determine the necessary safety criteria that all food products need to comply with. In this article, we discuss those related to the microbiological parameters of food.

Regulation (EC) No 178/2002 of the European Parliament and of the Council of 28 January 2002 laying down the general principles and requirements of food law, establishing the European Food Safety Authority and laying down procedures in matters of food safety, as amended, which is the basic piece of EU legislation formulating the principles and requirements of European food law, prohibits the placing on the market of food that is not safe. Food business operators are obliged to withdraw unsafe food from the market.

Commission Regulation (EC) No 2073/2005 of 15 November 2005 on microbiological criteria for foodstuffs, as amended, lays down harmonised safety criteria for the evaluation of food products as regards pathogenic micro-organisms to increase the protection of public health and to avoid divergent interpretations. This regulation and subsequent amending regulations establish harmonised food safety criteria for certain pathogenic micro-organisms. These criteria also serve to validate and verify HACCP procedures and other hygiene control measures.

In the light of the legal acts cited, a distinction is made between:

    1. food safety criteria, i.e. requirements determining the acceptability of a product or a batch of foodstuff, applied to products placed on the market. This group includes:
      • Salmonella (including serogroups Typhimurium and Enteritidis),
      • Listeria monocytogenes,
      • Cronobacter,
      • Escherichia coli,
      • Shiga-toxin producing E. coli (STEC) O157, O26, O111, O103, O145 and O104:H4,
      • Staphylococcal enterotoxins,
    2. process hygiene criteria, i.e. requirements for the acceptance of a functioning production process. They do not apply to products placed on the market. These criteria determine the indicative value of contaminants, exceeding which corrective actions are necessary to maintain the hygiene of the process at the level compliant with the food law. This group includes:
      • Aerobic bacteria count,
      • Enterobacteriacae,
      • Salmonella,
      • Escherichia coli,
      • Campylobacter spp.,
      • Coagulase-positive staphylococci,
      • Bacillus cereus.

The microbiological laboratories of J.S. Hamilton Poland offer comprehensive hazard monitoring at every stage of production, including environmental control, using the methodology specified in Polish standards, harmonised with European and ISO standards.

In addition to the tests included in the above criteria, we also perform the following microbiological analyses:

  • Bacillus subtilis,
  • Bacillus coagulans,
  • Listeria spp.,
  • Cronobacter spp.,
  • Alicyclobacillus spp.,
  • Moulds and yeasts,
  • Lactic acid bacteria,
  • Acetic acid bacteria,
  • Aerobic psychrotrophic microorganisms,
  • Spore-forming and non-spore-forming anaerobes, meso- and thermophilic,
  • Clostridium perfringens,
  • Clostridium botulinum,
  • Enterococcus,
  • Pseudomonas,
  • Characteristic bacteria in yoghurt,
  • Osmophilic moulds and yeasts,
  • Durability testing by thermostat test.

In addition to classical, standardised methods, J.S. Hamilton Poland laboratories have validated and accredited rapid alternative methods, based on the BAX® automated system based on the detection of a specific DNA sequence characteristic for pathogenic microorganisms. These methods are applicable to testing food, environmental samples from food production and trade areas. Using the BAX® system, we can determine the presence of such pathogens as:

  • Salmonella,
  • Listeria monocytogenes,
  • Escherichia coli O157:H7,

and for meat and meat products, vegetables, fruit and sprouts:

  • Shiga-toxin producing Escherichia coli (STEC) O157, O26, O111, O103, O145 oraz O104:H4.

As part of its services, J.S. Hamilton Poland also offers fast and precise detection of viruses present in ready-to-eat foods, which are not subjected to heat treatment during preparation and do not require heat treatment before consumption. These viruses mainly include:

  • Hepatitis A virus,
  • Noroviruses (also known as Norwalk viruses).

Using RT-PCR (reverse transcription PCR) we can determine their presence in matrices such as:

  • Fruit and processed fruit,
  • Vegetables and processed vegetables,
  • Environmental samples from the food production and marketing area.

Within the scope of accreditation, we also carry out microbiological analyses of drinking water, as well as tests of microbiological purity of packaging intended for contact with food.

Contact us today for more detail – or to schedule your tests at the microbiological labs of J.S. Hamilton.

 

GMO

Genetically modified food is food produced from and/or containing ingredients produced from GMOs. GMO food may only be authorised for sale if it has been confirmed that:

  • does not pose a danger to health;
  • does not mislead consumers;
  • has no less nutritional value than its non-GM counterpart.

Our laboratory at J.S. Hamilton Poland performs analyses of food and feed for the detection of genetic modifications in accordance with European law. Regulation (EC) No 1829/2003 of the European Parliament and of the Council of 22 September 2003 on genetically modified food and feed, as amended, requires the labelling of all food and feed products containing, consisting of or produced from GMOs. The threshold for GMO content mandating labelling is set at 0.9%. GMO content below this threshold is considered adventitious or technically unavoidable and does not have to be indicated on the label.

In the case of products containing or consisting of GMOs, this information must be indicated on the labels in accordance with Regulation (EC) No 1830/2003 of the European Parliament and of the Council of 22 September 2003. It is also possible, after complying with the requirements established in the Act of 13 June 2019 on the labelling of products produced without the use of genetically modified organisms as free from such organisms, to label food or feed as GMO-free. Graphic signs, which can be used in the labelling of GMO-free food and feed, are regulated by the Regulation of the Minister of Agriculture and Rural Development of 4 November 2019 on graphic signs, which are used for the purpose of labelling food and feed as free from genetically modified organisms.

Our laboratory at J.S. Hamilton Poland performs tests for the detection and quantification of GMOs in three steps, using the real-time PCR technique:

  • stage I – screening – this stage of testing for the presence of GMOs consists in detection of elements of genetic constructs most commonly used in the creation of GMO plants. At this stage the presence of the 35S promoter, NOS terminator and FMV promoter as well as pat, bar and EPSPS elements is detected,
  • stage II – qualitative testing for modification of soybean/hemp/corn – depending on the composition of the sample. In the J.S. Hamilton Poland laboratory we can perform the following qualitative GMO tests:
    • GMO soybean (RR, RR2, A2704-12, A5547-127),
    • GMO corn (BT11, BT176, T25, GA21, TC1507, MON810, MON88017),
    • Corn NK603 quality,
    • Corn MON89034 quality,
    • RT-73 rapeseed quality,
  • stage III – quantitative study including determination of GMO crops (RR soybean, RR2 soybean, RT-73 rapeseed, MON810 maize, NK603 corn).

 

Food packaging in Circular Economy

To tackle the climate crisis, the European Union seeks to promote the transition to a Circular Economy where products use less material, contain more recycled content and are easily recycled or reused. As part of this drive, the European Commission wants to ensure that “all packaging on the EU market is reusable or recyclable in an economically viable way by 2030”. The use of recycled plastics, especially as Food Contact Materials, is challenging but necessary due to a global convergence on concerns around the environmental impact of plastic. Sustainability objectives and the drive to a circular economy is an increasing demand for all recycled materials, and in particular plastics in packaging. Both, consumer demands and legislative changes have mandated a shift towards manufacturers reducing single-use plastics and the wider implementation of a circular economy.

WHAT ARE PCR MATERIALS?

PCR materials are recovered plastics that are recycled, i.e. re-processed. Mostly they originated from consumers, from used plastic packaging, which the consumer trash in the bin. The stream of such a waste includes: packaging from  food products, packaging from cosmetics, household chemicals or medicaments. Therefore, they are a mixture of such materials as: LDPE, HDPE, PP, PS, PET, laminates and other plastics.

WHY PCR MATERIALS?

Currently, the European Union takes the great emphasis great emphasis on increasing the usage of recycled plastics, including PCR plastics, for the production of packaging. The reason for this approach is the desire to reduce the amount of waste being landfilled and reduce the impact of the rainfall on the environment. According to the Single Use Plastic Directive of 2019. By 2025, PET beverage bottles are to contain at least 25% recycled plastic. From 2030, PET beverage bottles are to contain at least 30% recycled plastics. At the same time, the market of packaging for food and cosmetic products strives to produce packaging with the highest possible share of PCR material. This is a trend that has already started from for several years. Packaging made of 100% PCR is already available on the market.

SAFETY PCR MATERIALS AS FCMS

In accordance with the Regulation (EC) 1935/2004 each food contact material must be safe for the final recipient, i.e. the consumer, under foreseeable conditions of use. Regulation (EU) 1934/2004 defines this safety as:

  • no chemical release above safe limits,
  • no change in food composition,
  • no change in the organoleptic characteristics of the food.

Recycled plastics intended to be used in contact with food should also meet the requirements of the Commission Regulation (EC) No. 282/2008. The Work is currently underway to amend this regulation. The current legal status requires the use of PCR plastics behind a functional barrier. The above requirements can also be applied to packaging intended for cosmetics. The packaging manufacturer must ensure constant supervision of the entire production process and the composition in the final product. For this reason, there are additional risks associated with the use of PCR plastics. The most important is the possibility of contamination of the main material with admixtures of other materials. The final product may contain substances that are not allowed to come into direct contact with food, such as printing inks, adhesives, varnishes, coatings. PCR materials may contain substances that are commonly considered harmful and undesirable, such as: bisphenols, phthalates, heavy metals, SVHC substances and others. It should be remembered that production batches may differ significantly from each other.

The safety of food contact materials requires evaluation as chemicals can migrate from the materials into food. The materials should be manufactured in compliance with EU regulations, including good manufacturing practices, so that any potential transfer to foods does not raise safety concerns, change the composition of the food in an unacceptable way or have adverse effects on the quality of foods (for instance, taste and/or odour). For this reason, the scope and frequency of tests should be significantly increased. J.S. Hamilton Poland offers the wide range of analyzes to ensure the safety of the produced PCR materials is achieved.

At J.S. Hamilton, we offer the following services related to food packaging:

  • Consultancy on PCR materials as FCMs
  • Set-up of optimized testing plans for compliance verification of PCR
  • Documents review, DoCs for raw materials verification
  • Compliance assessment based on EU 10/2011
  • Toxicological risk assessment e.g. by TTC-concept method
  • Overall and specific migration for compliance with EU 10/2011
  • Non-Intentionally Added Substances (NIAS):
    • Headpsace-GC/MS Screening,
    • GC/MS Screening,
    • LC-QToF-MS Screening,
    • ICP-MS Screening,
    • Primary Aromatic Amines (PAAs).
  • Testing of hazarduos substances:
    • Metals
    • Bisphenols
    • Phthalates
    • Alkylphenols
    • Perfluorinated alkyl compounds (PFAS.)
    • Chlorinated paraffins (LCCP, MCCP, SCCP)
    • Mineral oils
    • Polycyclic Aromatic Hydrocarbons (PAHs)
    • Photoinitiators, acrylates and other compounds of printing inks
    • Polybrominated biphenyls (PBB) and polybrominated diphenyl ethers (PBDE)
    • Organotin compounds
    • Other SVHC substances

Contact our team today to learn more about food packaging tests and the demands of circular economy that your business might wish to satisfy.